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Alpha cat regular test kit

Alpha cat regular test kit



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Alpha cat regular test kit (Cat# EK-CAT R-G-P). The intra- and interassay CVs for the assay were 3.8% and 9.7%, respectively. Plasma levels of testosterone and DHEAS were measured using commercial ELISA kits (Cat# DIAsource K010-30 and K067-12, respectively). The intra- and interassay CVs for testosterone and DHEAS were 8.2% and 6.7% and 8.5% and 6.3%, respectively. A multiplexed bead array (Luminex, EMD Millipore, Billerica, MA) was used to quantify serum samples for C-reactive protein (CRP), matrix metalloproteinase-9 (MMP-9), placental growth factor (PlGF), vascular endothelial growth factor (VEGF), and soluble fms-like tyrosine kinase-1 (sFlt-1). Samples were read and analyzed using a Bio-Plex 200 system and Bio-Plex Manager version 6.1 software (Bio-Rad Laboratories, Inc.). The intra- and interassay CVs for all these assays were ≤5.5%. Circulating CRP levels were quantified using a commercial ELISA kit (Immunodiagnostic Systems). The intra- and interassay CVs for CRP were 5.4% and 7.7%, respectively. Circulating MMP-9 and PlGF levels were measured using Milliplex Map kit (EMD Millipore). The intra- and interassay CVs for MMP-9 and PlGF were ≤12.1%. Circulating VEGF and sFlt-1 levels were measured using the Quantikine ELISA kit (R&,D Systems). The intra- and interassay CVs for VEGF and sFlt-1 were ≤4.1% and 5.7%, respectively.

Statistical analysis

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Patient characteristics, angiogenic factors, and obstetric and neonatal outcome parameters were analyzed using the Student's *t*-test for continuous variables or Fisher's exact test for categorical variables. Because PlGF and VEGF measurements were skewed, data were natural log transformed. Multivariable linear regression models were used to evaluate the associations between angiogenic factor levels and maternal and fetal outcomes. The following angiogenic factors were included in all models: CRP, MMP-9, PlGF, VEGF, and sFlt-1. Because of the possibility that there may be residual confounding in the relationship between angiogenic factors and preeclampsia, a sensitivity analysis was performed in which patients with mild and severe preeclampsia were combined. A *P*-value <,0.05 was considered statistically significant. All tests were 2-sided. Statistical analyses were performed using SAS software (version 9.1, SAS Institute, Inc, Cary, NC).

Results

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Study population

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Of the 160 patients who consented to participate, data were avlable from 153 patients (median gestational age at blood sample collection, 24.6 weeks, range, 22.0 to 28.9 weeks). The majority of participants (n = 95 [61.3%]) were African American and 35.9% were of Black/African descent. Maternal age ranged from 19 to 41 years (median age, 26 years). The majority of patients had ≥1 comorbidities, including gestational diabetes mellitus, chronic hypertension, and/or asthma (Table [1](#T1){ref-type="table"}). The demographic and clinical characteristics of the 153 women with complete data are summarized in Table [2](#T2){ref-type="table"}.

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Demographics and clinical characteristics of women by ethnicity

 , **Non-Hispanic White (n = 33)** **African American (n = 35)** **Hispanic (n = 17)**

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Maternal age at blood collection (years)  ,  ,  ,

<, 20 0 (0.0%) 1 (0.8%)  ,  ,

20-24 10 (6.4%) 8 (6.3%)  ,  ,

  25-29 29 (18.6%)